Journal: Oncotarget

Article Title: Tumor suppressor REIC/DKK-3 and co-chaperone SGTA: Their interaction and roles in the androgen sensitivity

doi: 10.18632/oncotarget.6488

Figure Lengend Snippet: A. The yeast two-hybrid analysis was conducted using pPC86 (AD)/full-length human SGTA (derived from a normal heart cDNA library) and pDBLeu (BD)/full-length human REIC/DKK-3 plasmids. The blue colonies indicate those with an interaction between the two proteins. B. For the pull-down (PD) assay, the full-length cDNA of human REIC/DKK-3 and SGTA was cloned into the pFN21A and pMACS Kk.HA-C plasmids, respectively. Cell lysates from Halo-tagged REIC/DKK-3- and/or HA-tagged SGTA-transfected 293T cells were analyzed. The sample pulled down using Halo-tagged REIC/DKK-3 was analyzed by Western blotting (WB) using anti-HA antibody. C. REIC/DKK-3 and SGTA protein expression in 293T, PC3 and Hs68 cells was analyzed by Western blotting. Coomassie Brilliant Blue (CBB) staining of the membrane is shown as a loading control. D. The co-localization of REIC/DKK-3 and SGTA was examined by double immunofluorescence staining and observed by fluorescence microscopy. The images in green and red show the intracellular localization of REIC/DKK-3 and SGTA, respectively. The areas of overlap between REIC/DKK-3 and SGTA are shown in yellow in the merged image.

Article Snippet: The normal human foreskin fibroblast cell line Hs68 was provided by JCRB Cell Bank (Osaka, Japan).

Techniques: Derivative Assay, cDNA Library Assay, Clone Assay, Transfection, Western Blot, Expressing, Staining, Membrane, Control, Double Immunofluorescence Staining, Fluorescence, Microscopy

Journal: Journal of the Society of Cosmetic Scientists of Korea

Article Title: The Cosmeceutical Property of Antioxidant Astaxanthin is Enhanced by Encapsulation Using Glyceryl Based New Vesicle

doi: 10.15230/scsk.2014.40.3.247

Figure Lengend Snippet: Figure 3. 2D-PAGE image from human fibroblast(control).

Article Snippet: Human dermal fibroblast cells (ATCC, CRL-1635, American Type Culture Collection, 249글리세릴 베이스의 신규베지클 이용 캡슐화를 통한 항산화성 아스타잔틴의 성질 강화 J. Soc.

Techniques: Control

Journal: Journal of the Society of Cosmetic Scientists of Korea

Article Title: The Cosmeceutical Property of Antioxidant Astaxanthin is Enhanced by Encapsulation Using Glyceryl Based New Vesicle

doi: 10.15230/scsk.2014.40.3.247

Figure Lengend Snippet: Figure 4. 2D-PAGE image from human fibroblast. Cell treated with AX. Extract.

Article Snippet: Human dermal fibroblast cells (ATCC, CRL-1635, American Type Culture Collection, 249글리세릴 베이스의 신규베지클 이용 캡슐화를 통한 항산화성 아스타잔틴의 성질 강화 J. Soc.

Techniques:

Journal: Journal of the Society of Cosmetic Scientists of Korea

Article Title: The Cosmeceutical Property of Antioxidant Astaxanthin is Enhanced by Encapsulation Using Glyceryl Based New Vesicle

doi: 10.15230/scsk.2014.40.3.247

Figure Lengend Snippet: Figure 2. Cell toxicity of fibroblast treated with AX extract.

Article Snippet: Human dermal fibroblast cells (ATCC, CRL-1635, American Type Culture Collection, 249글리세릴 베이스의 신규베지클 이용 캡슐화를 통한 항산화성 아스타잔틴의 성질 강화 J. Soc.

Techniques:

Journal: Cancer research

Article Title: Targeting histone demethylases in MYC-driven neuroblastomas with ciclopirox

doi: 10.1158/0008-5472.CAN-16-0826

Figure Lengend Snippet: (A) Cells were treated with ciclopirox for 96 hours. Curves showing that ciclopirox suppresses viability of neuroblastoma cells but not HS68 cells, a normal human fibroblast cell line. The IC50 of CPX to each cell line was summarized in the table. The proliferation index for each cell line was determined and summarized in the table.

Article Snippet: Human 293T, HS68, BE(2)-C, SK-N-AS, IMR-32, SK-N-SH, and U2OS cell lines were purchased from the American Type Culture Collection (ATCC) between 2012 to 2014.

Techniques: