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JCRB Cell Bank
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ATCC
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BioResource International Inc
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ATCC
materials human foreskin fibroblasts ![]() Materials Human Foreskin Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/normal+human+dermal+fibroblast+hs68+cell/pm37695167-328-0-5?v=ATCC Average 96 stars, based on 1 article reviews
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SACRI Antibody
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Santa Cruz Biotechnology
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European Collection of Authenticated Cell Cultures
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Image Search Results
Journal: Evidence-based Complementary and Alternative Medicine : eCAM
Article Title: Antimetastatic Potentials of Dioscorea nipponica on Melanoma In Vitro and In Vivo
doi: 10.1155/2011/507920
Figure Lengend Snippet: The effects of DNE3 on cell viability, migration, motility, invasion, and adhesion of melanoma cells. (a) B16F10 and (b) HS68 cells were treated with DNE3 for 24 hours by MTT assay. (c) B16F10 cells were subjected to analyze for cell migration by wound healing assay. (d) Determined migration ability of B16F10 was subsequently quantified with that of control being 100% (without DNE3 for 48 h). Cells were treated with DNE3 for 24 hours and then subjected to analyze for (e and f) motility, invasion, and (g) adhesion as described in Materials and Methods. Results were statistically evaluated by using one-way ANOVA with post hoc Dunnett's test (*** P < .001). Results from 3 repeated and separated experiments were similar.
Article Snippet: Cell lines including A2058 from human melanoma, B16F10 from a mouse melanoma, and
Techniques: Migration, MTT Assay, Wound Healing Assay, Control
Journal: Oncotarget
Article Title: Tumor suppressor REIC/DKK-3 and co-chaperone SGTA: Their interaction and roles in the androgen sensitivity
doi: 10.18632/oncotarget.6488
Figure Lengend Snippet: A. The yeast two-hybrid analysis was conducted using pPC86 (AD)/full-length human SGTA (derived from a normal heart cDNA library) and pDBLeu (BD)/full-length human REIC/DKK-3 plasmids. The blue colonies indicate those with an interaction between the two proteins. B. For the pull-down (PD) assay, the full-length cDNA of human REIC/DKK-3 and SGTA was cloned into the pFN21A and pMACS Kk.HA-C plasmids, respectively. Cell lysates from Halo-tagged REIC/DKK-3- and/or HA-tagged SGTA-transfected 293T cells were analyzed. The sample pulled down using Halo-tagged REIC/DKK-3 was analyzed by Western blotting (WB) using anti-HA antibody. C. REIC/DKK-3 and SGTA protein expression in 293T, PC3 and Hs68 cells was analyzed by Western blotting. Coomassie Brilliant Blue (CBB) staining of the membrane is shown as a loading control. D. The co-localization of REIC/DKK-3 and SGTA was examined by double immunofluorescence staining and observed by fluorescence microscopy. The images in green and red show the intracellular localization of REIC/DKK-3 and SGTA, respectively. The areas of overlap between REIC/DKK-3 and SGTA are shown in yellow in the merged image.
Article Snippet: The normal
Techniques: Derivative Assay, cDNA Library Assay, Clone Assay, Transfection, Western Blot, Expressing, Staining, Membrane, Control, Double Immunofluorescence Staining, Fluorescence, Microscopy